raf1 (Santa Cruz Biotechnology)

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Structured Review

Santa Cruz Biotechnology raf1
Raf1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 911 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raf1/Raf-1+Antibody/pm41664072-93-21-24
Average 94 stars, based on 911 article reviews

raf1 - by Bioz Stars, 2026-10

94/100 stars

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Ubiquitin Proteomics:

Article Title: USP10-mediated deubiquitination and activation of KRAS mutants promotes colorectal cancer via a novel USP10/KRAS positive feedback circuit
Article Snippet: Antibodies against HA (#db2603, RRID: AB_3451976) and GAPDH (#db106, RRID: AB_3271576) were purchased from Diagbio Biosciences (Hangzhou, China). .. Antibodies against KRAS (#sc-30, RRID: AB_627865), ubiquitin (Ub) (#sc-8017, RRID: AB_628423), and RAF1 (#sc-7267, RRID: AB_628196) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). .. Antibodies against MYC (#A00704, RRID: AB_914461) and FLAG (#A00187, RRID: AB_1720813) were obtained from GenScript (Nanjing, China).

Article Title: USP10-mediated deubiquitination and activation of KRAS mutants promotes colorectal cancer via a novel USP10/KRAS positive feedback circuit
Article Snippet: Antibodies against HA (#db2603, RRID: AB_3451976 ) and GAPDH (#db106, RRID: AB_3271576 ) were purchased from Diagbio Biosciences (Hangzhou, China). .. Antibodies against KRAS (#sc-30, RRID: AB_627865 ), ubiquitin (Ub) (#sc-8017, RRID: AB_628423 ), and RAF1 (#sc-7267, RRID: AB_628196 ) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). ..

Western Blot:

Article Title: Non-canonical integrin signaling activates EGFR and RAS-MAPK-ERK signaling in small cell lung cancer.
Article Snippet: .. Western blotting was performed using standard methods and antibodies specific for 6x-HIS-Tag (Abcam), GFP (Santa Cruz), ITGB2 (R&D Systems), ITGB6 (R&D Systems), ITGA2 (R&D Systems), pMAPK (Cell Signaling), MAPK (Cell Signaling), pEGFR (Cell Signaling), EGFR (Cell Signaling), GAL3 (Santa Cruz), KRAS (Santa Cruz), pRAF1 (Cell signaling), RAF1 (Cell signaling), pERK (Cell signaling), ERK (Cell signaling), MYCN (Santa Cruz), TSG101 (Santa Cruz), CD63 (Santa Cruz), FN1 (Millipore), ACTA2 (Sigma), VIM (Cell Signaling), NKX2-1 (Santa Cruz), EZH2 (Abcam), H3K27me3 (Millipore), ASH-1 (Chemicon), TP53 (Cell signaling) and GAPDH (Sigma) were used. .. Immunoreactive proteins were visualized with the corresponding HRP-conjugated secondary antibodies (Santa Cruz) using the Super Signal West Femto detection solutions (ThermoFisher Scientific).

Article Title: Cell-specific models reveal conformation-specific RAF inhibitor combinations that synergistically inhibit ERK signaling in pancreatic cancer cells.
Article Snippet: .. Basal level of RAS signaling protein: Pan-RAS (ab52939, Abcam), ARAF (#4432, CST), BRAF (F-7, sc-5284, Sant Cruz), RAF1 (E-10, sc-7267, Santa Cruz) total MEK (#9122, CST), pMEK1/2 (Ser217/221, #9121, CST), total ERK (Erk1/2, #9102, CST), pERK1/2 (M8159, Sigma-Aldrich), pAKT (Ser473, #9271, CST), pAKT(Thr308, #9275, CST) and total AKT (#9272, CST), was measured by Western Blot and normalized to GAPDH (#2118, CST). ..

Article Title: Cell-specific models reveal conformation-specific RAF inhibitor combinations that synergistically inhibit ERK signaling in pancreatic cancer cells
Article Snippet: .. Basal level of RAS signaling protein: Pan-RAS (ab52939, Abcam), ARAF (#4432, CST), BRAF (F-7, sc-5284, Sant Cruz), RAF1 (E-10, sc-7267, Santa Cruz) total MEK (#9122, CST), pMEK1/2 (Ser217/221, #9121, CST), total ERK (Erk1/2, #9102, CST), pERK1/2 (M8159, Sigma-Aldrich), pAKT (Ser473, #9271, CST), pAKT(Thr308, #9275, CST) and total AKT (#9272, CST), was measured by Western Blot and normalized to GAPDH (#2118, CST). ..

Cell-Signaling:

Article Title: Discovery of KRB-456, a KRAS G12D Switch-I/II Allosteric Pocket Binder That Inhibits the Growth of Pancreatic Cancer Patient-derived Tumors
Article Snippet: .. The following antibodies were used: Phospho-MEK1/2 (S217/221; catalog no. 9154S), MEK1/2 (catalog no. 4694S), Phospho-AKT (S473; catalog no. 9271S), Phospho-AKT (T308; catalog no. 4056S), AKT (catalog no. 9272S), Phospho-Erk1/2 (catalog no. 4370S), Erk1/2 (catalog no. 4695S), Phospho-S6 Ribosomal Protein (Ser 235/236; catalog no. 4858S), S6 Ribosomal Protein (catalog no.2217S), cleaved-CASP-3 (catalog no. 9664), cleaved-PARP (catalog no. 9541) from Cell Signaling Technology; anti-β-ACTIN (catalog no A5441-.2ML from Sigma-Aldrich; KRAS (catalog no. OP24, from EMDMillipore), and RAF1 (catalog no. sc-7267, Santa Cruz Biotechnology), horseradish peroxidase–conjugated goat anti-GST antibody (catalog no. A190-121P from Bethyl Laboratories). .. Cell viability assays were carried out using the CellTiter-Glo Luminescent Cell Viability Assay (Promega).

Incubation:

Article Title: GPR35 protects against reperfusion injury in ischemic stroke by binding with the CR2 domain of Raf1.
Article Snippet: .. Sections were incubated with NeuN (1:100, 94403, CST), Iba1 (1:100, MA527726, Invitrogen), GFAP (1:200, GB12096-100, Servicebio), CD68 (1:200, ab283654, Abcam), or Raf1 (1:100, sc7267, Santa Cruz), followed by DyLight-conjugated secondary antibodies (1:200, E032210-01, E032420-01, EarthOx) and DAPI counterstaining. ..

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